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Cytotoxicity Assay : ISO 10993

Cytotoxicity and cell viability is one of the most important indicators for biological evaluation in vitro studies. Chemicals such as drugs and pesticides have different cytotoxicity mechanisms such as destruction of cell membranes, prevention of protein synthesis, irreversible binding to receptors etc. In order to determine the cell death caused by these damages, the following sutable assays can be performed .

  • Cell functional assays: ATP, ADP, ATP/ADP ratios.
  • Cytology/Membrane leakage assays: LDH and AK test.
  • Mitochondrial assays: MTT, MTS, XTT.
  • DNA damage assays: BrdU test.